Physiology & Biophysics

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GE, AKTA Explorer A100 FPLC with MALS and RI detector



Manager: Yinghua Chen, PhD
Contact Email: yinghua.chen@case.edu
Contact Phone: 216-368-8962
Availablility: Sign Up

 

Major Applications:

FPLC is used for analysis or purification of mixture of proteins; The combination of FPLC with UV and MALS (Treos and optilab rEX) allows the determination of the molecular weight and the amount of aggregation.

Major Specifications

Flow rate: 100 ml/min

Pressure limit: 10 MPa

Multiple wavelength detection: 190-700 nm, up to 3 wavelengths simultaneously

Conductivity monitoring: 1µS/cm-999 µS/cm

Buffer selection: 8 buffers

Reversed flowthrough column: standard

Automated sample injection: standard

Multiple sample injection: 8 samples

Fraction collector: Frac-950


Manual: Amersham AKTA Explorer 100 FPLC User Guide
Manufacturer: GE healthcare (Amersham Pharmacia)
Location: SOM-E549
Required Training: Basic Training
Training Fee: 100.00

Usage Fees:
AKTA FPLC explorer 100:$10.00 Per hour
AKTA FPLC explorer 100 with MALS and RI detector:$26.00 Per hour
MALS (miniDAWN):$8.00 Per hour
RI detector (Optilab rEX) :$8.00 Per hour

Consumable Fees:
Superdex 200 10/300 GL:$10.00 per sample run
HiPrep 16/60 Sephacryl S-100 high resolution:$10.00 per sample run
Hiload 26/60 superdex 200 prep grade:$10.00 per sample run
Hiload 16/60 superdex 75 prep grade:$10.00 per sample run
Superdex 75 10/300 GL:$10.00 per sample run
Director: Matthias Buck, MA, DPhil
Owner: Walter F. Boron, MD, PhD
Responsible PI: Walter F. Boron, MD, PhD
Class: Core Equipment -- PEPCMBC