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Tingwei Mu, PhD
Associate Professor
PhD, Biochemistry, California Institute of Technology
View Curriculum Vitae (pdf)

Mailing Address:
Robbins E513
Phone: 216-368-0750
Fax: 216-368-5586

Research Interests

Proteostasis maintenance of membrane proteins in health and disease

  • We welcome graduate students to join the laboratory for rotations and thesis work.

My laboratory aims to understand protein homeostasis (proteostasis) of ion channels. They are major drug targets; loss of their proteostasis and thus function leads to numerous diseases, including neurological, neurodegenerative, and cardiovascular diseases. To function, ion channel proteins need to fold into their native structures and assemble properly in the endoplasmic reticulum (ER) for subsequent trafficking to the plasma membrane in a fully functional state. Mutations in a given protein could lead to protein misfolding and excessive ER-associated degradation (ERAD), and thus a significantly lowered concentration of proteins in cell membranes and loss of function.

Currently, in my laboratory, we focus on studying gamma-aminobutyric acid type A (GABAA) receptors. They are the primary inhibitory ion channels in the mammalian central nervous system. Loss of their function leads to epilepsies, autism, and other neurodevelopment diseases.

We explore how molecular chaperones, folding enzymes, ERAD factors, and trafficking factors, coordinate to facilitate membrane protein folding, assembly, degradation, and trafficking.

We also use small molecule proteostasis regulators to correct misfolded membrane proteins, as a therapeutic strategy to treat corresponding diseases.

Proteostasis maintenance of GABAA receptors

Ion channel proteins maintain a delicate balance between protein synthesis, folding, assembly, trafficking and degradation. Mutant membrane proteins are subjected to excessive endoplasmic-reticulum associated degradation. Molecular chaperones and ERAD factors play a key role in regulating their proteostasis.

Specific Projects
  1. Understanding the molecular mechanism underlying ion channel folding, assembly and degradation

    Because the activity of membrane proteins is coupled to other proteins in complicated cellular interaction networks, the molecular mechanism of ion channel folding and degradation in the cell has been elusive. Using representative membrane proteins, we aim to reveal the protein homeostasis network that control their folding, assembly, degradation, and trafficking and thus function using proteomics, cell biology, electrophsiology, and animal model approaches.

  2. Ameliorating ion channel misfolding diseases

    We focus on diseases resulting from protein misfolding and thus reduced membrane protein surface expression and compromised function. This defective protein trafficking has been reported to result in many diseases, including neurodegenerative diseases and cardiovascular diseases.

    We focus on developing multiple strategies to discover small molecules that enhance proper protein folding in cells to ameliorate diseases. We also explore the mechanism of action of these small molecules: how they alter the signaling pathways associated with the protein homeostasis network for disease intervention. Ultimately, we aim to develop novel therapeutic strategies for diseases associated with protein misfolding.

Featured Publications
  • Wang YJ, Vu GH, Mu TW (2023) Pathogenicity Prediction of GABAA Receptor Missense Variants.  bioRxiv, doi: PMID: 38014242. Israel Journal of Chemistry, doi: 10.1002/ijch.202300161.
  • Wang YJ, Seibert H, Ahn LY, Schaffer AE, Mu TW (2023) Pharmacological chaperones restore proteostasis of epilepsy-associated GABAA receptor variants. bioRxiv, doi: PMID: 37131660
  • Zhang PP, Benske TM, Ahn LY, Schaffer AE, Paton JC, Paton AW, Mu  TW, Wang YJ (2023) Adapting the endoplasmic reticulum proteostasis rescues epilepsy-associated NMDA receptor variants. Acta Pharmacologica SinicaDOI: 10.1038/s41401-023-01172-w. PMID: 37803141
  • Wang YJ, Di XJ, Han DY, Nashmi R, Henderson BJ, Moss FJ, Mu TW (2022) Hsp47 Promotes Biogenesis of Multi-subunit Neuroreceptors in the Endoplasmic Reticulum. bioRxiv, doi:
  • Benske T, Mu TW, Wang YJ (2022) Protein Quality Control of N-methyl-D-aspartate Receptors. Front. Cell. Neurosci. - Cellular Neurophysiology, DOI: 10.3389/fncel.2022.907560. PMID: 35936491
  • Wang M, Cotter E, Wang YJ, Fu X, Whittsette AL, Lynch JW, Wiseman RL, Kelly JW, Keramidas A, Mu TW (2022). Pharmacologic ATF6 Activation Remodels the Proteostasis Network to Rescue Pathogenic GABAA Receptors. Cell & Bioscience. 12, 48. PMID: 35477478
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