Molecular mechanisms of membrane remodeling, fission and fusion in endocytosis and mitochondrial dynamics
Molecular Biophysics of Membrane Remodeling in Membrane Fission and Fusion
Cellular membranes undergo dynamic changes in shape in remarkably striking ways during processes such as cell movement and division, and also during vesicle fission and fusion events integral to intracellular membrane trafficking. Dynamins are a family of large GTPases involved in synaptic vesicle recycling, clathrin-mediated endocytosis, budding of intracellular transport vesicles, mitochondrial division/fusion, and peroxisome biogenesis. They often work in concert with membrane-sculpting BAR domain-containing proteins and various accessory molecules in effecting membrane fission and fusion. The main objective of our research is to reconstitute and elucidate the various molecular machineries and mechanisms involved in intracellular membrane remodeling, fusion and fission, specifically in synaptic vesicle endocytosis and mitochondrial dynamics, using cutting-edge state-of-the-art fluorescence spectroscopic techniques, including single-molecule FRET, FCS, and related biophysical techniques.
- Ramachandran R, TJ Pucadyil, YW Liu, S Acharya, M Leonard, V Lukiyanchuk & SL Schmid.Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission. Mol. Biol. Cell 20:4630-9, 2009.
- Ramachandran R & SL Schmid.Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membrane. EMBO J. 27:27-37, 2008.