PEPCMBC Equipment

Protein Expression & Purification

• Incubators

  • Bellco, Insect Cell Incubator
    • Barnstead, Stir Plates (7)
    • Corning, Spinner Flasks
  • Thermo, Max Q5000 Floor Shaking Incubator (Bacteria))
  • Thermo, Max Q5000 Floor Shaking Incubator (Yeast)

• Fermentor & Bioreactor

  • Eppendorf, BioFlo/Celligen-115

• Sonicator

  • Branson, Sonifer

• High Speed Centrifuge

  • Beckman, Avanti JXN-26 Centrifuge
    • Beckman, Avanti J26XPI Centrifuge
    • Beckman, JLA-8.1000 Rotor

• FPLC

  • GE, AKTA Explorer A100 FPLC with MALS and RI detector
    • Wyatt, Dynamic Light Scattering (DynoPro NanoStar)
    • Wyatt, Multi-angle Light Scattering (miniDAWN TREOS)
    • Wyatt, Optilab rEX (RI detector)

• Spectrophotometer

  • Beckman, DU-640 Spectrophotometer

• Water Purifcation System

  • Siemens, Modulab Water Purification System

• Biological Safety Cabinet

  • Thermo-Fisher, Biological Safety Cabinet (1300 Series Class II, Type A2)

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Protein Biophysics

• Protein Characterization

  • AVIV, Circular Dichroism Spectrometer
  • Biacore, T200
  • Microcal, Isothermal Titration Calorimeter (ITC200)
  • Monolith NT.115 Microscale Thermophoresis (Nano Temper Technologies)
  • Tecan Spark
    • Tecan, Infinite M1000 Quadruple Monochromator Microplate Reader

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Automated Crystallization

• Liquid Handlers

  • HydraDT - Liquid Handler
  • Mosquito - Liquid Handler/Spotter
  • Tecan, Freedom Evo - Liquid Handler

• Protein Crystal Chambers

  • Formulatrix, Rock Imager 500
  • Powers, Protein Crystallography Incubators (2)

• Plate Sealer

  • Thermo, ALPS 50V - Manual Heat sealer

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Imaging

• Microscopes

  • Leica, M205A Stereomicroscope
  • Leica, S8 APO

• Image Aquisition & Documentation System

  • GE, Typhoon Imager

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